Nebulin Antibody (NB2)

Novus Biologicals | Catalog # NB120-11099

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Alligator, Amphibian, Avian, Bovine, Chicken, Fish, Rabbit

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NB2
View all Nebulin Primary Antibodies »

Product Specifications

Immunogen

Full length native protein (purified) (Chicken).

Reactivity Notes

Cross-reacts with a wide range of species, including salamander, pigeon, alligator, toad. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nebulin Antibody (NB2)

Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099]

Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099]

Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.

Applications for Nebulin Antibody (NB2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Western Blot

1:100-1:2000
Application Notes
IF: Use at a dilution of 1/400. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 um from the z-line in electron micrographs. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nebulin

Nebulin is a high molecular weight protein (500-800 kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched.

Alternate Names

DKFZp686C1456, FLJ11505, FLJ36536, FLJ39568, FLJ39584, NEB177D, nebulin, NEM2

Entrez Gene IDs

4703 (Human); 17996 (Mouse); 311029 (Rat)

Gene Symbol

NEB

Additional Nebulin Products

Product Documents for Nebulin Antibody (NB2)

Certificate of Analysis

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Product Specific Notices for Nebulin Antibody (NB2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nebulin Antibody (NB2)

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Protocols

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