Nectin-1/PVRL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12960

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Canine (95%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Nectin-1/PVRL1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Nectin-1/PVRL1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Nectin-1/PVRL1 Antibody [NBP3-12960]

Immunocytochemistry/ Immunofluorescence: Nectin-1/PVRL1 Antibody [NBP3-12960]

Immunocytochemistry/Immunofluorescence: Nectin-1/PVRL1 Antibody [NBP3-12960] - Nectin-1/PVRL1 antibody detects Nectin-1/PVRL1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nectin-1/PVRL1 protein stained by Nectin-1/PVRL1 antibody (NBP3-12960) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Nectin-1/PVRL1 Antibody - BSA Free

Nectin-1/PVRL1 Antibody [NBP3-12960] -

Nectin 1 antibody detects Nectin 1 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nectin 1 stained by Nectin 1 antibody diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.
Nectin-1/PVRL1 Antibody - BSA Free

Western Blot: Nectin-1/PVRL1 Antibody [NBP3-12960] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 antibody (NBP3-12960) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Nectin-1/PVRL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-1

The PVRL1 gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobu

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Gene Symbol

NECTIN1

Additional Nectin-1 Products

Product Documents for Nectin-1/PVRL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nectin-1/PVRL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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