Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 110 expressed in HEK293

Format

Azide and BSA Free
View all Nectin-2/CD112 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Nectin-2/CD112 (Accession#: NP_033016.3; Met 1-Gly 351).

Specificity

No cross-reactivity in ELISA with: Human CD112 / Nectin-2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530]

Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530]

Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa
Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530]

Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530]

Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Mouse Nectin-2/CD112 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 0.5 ul anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa

Applications for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-2/CD112

Nectin 2 encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Gene Symbol

NECTIN2

Additional Nectin-2/CD112 Products

Product Documents for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Nectin-2/CD112 Antibody (110) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Nectin-2/CD112 Antibody (110) - Azide and BSA Free and earn rewards!

Have you used Nectin-2/CD112 Antibody (110) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies