Nectin-4/PVRL4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82829

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nectin-4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Nectin-4/PVRL4 Antibody - BSA Free (NBP1-82829) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nectin-4/PVRL4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining in human skin and skeletal muscle tissues.. Corresponding NECTIN4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunocytochemistry/ Immunofluorescence: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunocytochemistry/Immunofluorescence: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829]

Immunohistochemistry-Paraffin: Nectin-4/PVRL4 Antibody [NBP1-82829] - Staining of human skin shows moderate membranous positivity in keratinocytes.

Applications for Nectin-4/PVRL4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-4

PVRL4, also known as Poliovirus receptor-related protein 4, is a 510 amino acid protein that is 55 kDa, predominantly expressed in placenta and breast carcinoma, is a single-pass type I membrane protein involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1, and oes not act as receptor for alpha-herpesvirus entry into cells. Disease research is being performed in relation to this protein and ectodermal dysplasia, syndactyly, ectodermal dysplasia-syndactyly syndrome 1, measles, ovarian cancer, breast carcinoma, lung cancer, and carcinoma. The PVRL4 protein has shown an interaction with PVRL1, MLLT4, PICK1, TIGIT, PARD3, PVRL3, and PVRL2 in Adherens junctions interactions, Nectin/Necl trans heterodimerization, Cell junction organization, Cell-Cell communication, Cell-cell junction organization, Sertoli-Sertoli Cell Junction Dynamics, and Epithelial Adherens Junctions pathways.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Gene Symbol

NECTIN4

Additional Nectin-4 Products

Product Documents for Nectin-4/PVRL4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nectin-4/PVRL4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nectin-4/PVRL4 Antibody - BSA Free

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Protocols

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FAQs

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