NEDD4L Antibody (1D2) - Azide and BSA Free

Novus Biologicals | Catalog # H00023327-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D2

Format

Azide and BSA Free
View all NEDD4L Primary Antibodies »

Product Specifications

Immunogen

NEDD4L (AAH32597, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLT

Reactivity Notes

Human. Other species not tested.

Specificity

NEDD4L (1D2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NEDD4L Antibody (1D2) - Azide and BSA Free

Western Blot: NEDD4L Antibody (1D2) [H00023327-M04]

Western Blot: NEDD4L Antibody (1D2) [H00023327-M04]

Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04]

Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04]

Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.
Sandwich ELISA: NEDD4L Antibody (1D2) [H00023327-M04] - Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.

Applications for NEDD4L Antibody (1D2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEDD4L

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK

Alternate Names

E3 ubiquitin-protein ligase NEDD4-like, EC 6.3.2, EC 6.3.2.-, FLJ33870, hNedd4-2, KIAA0439ubiquitin-protein ligase NEDD4-like, NEDD4.2, Nedd4-2, NEDL3, neural precursor cell expressed, developmentally down-regulated 4-like, neural precursor cell expressed, developmentally down-regulated gene 4-like, RSP5ubiquitin-protein ligase Rsp5

Entrez Gene IDs

23327 (Human)

Gene Symbol

NEDD4L

OMIM

606384 (Human)

UniProt

AAH32597 (Human)

Additional NEDD4L Products

Product Documents for NEDD4L Antibody (1D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NEDD4L Antibody (1D2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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