Nesprin-3 Antibody (5P10J3)

Novus Biologicals | Catalog # NBP3-33295

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 5P10J3
View all Nesprin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 750-935 of human Nesprin-3 (NP_689805.3).

Sequence:
LIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLFRRACCVALPLQLLLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nesprin-3 Antibody (5P10J3)

Nesprin-3 Antibody (5P10J3)

Western Blot: Nesprin-3 Antibody (5P10J3) [NBP3-33295] -

Western Blot: Nesprin-3 Antibody (5P10J3) [NBP3-33295] - Western blot analysis of lysates from Mouse spleen, using Nesprin-3 Rabbit mAb at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Nesprin-3 Antibody (5P10J3)

Western Blot: Nesprin-3 Antibody (5P10J3) [NBP3-33295] -

Western Blot: Nesprin-3 Antibody (5P10J3) [NBP3-33295] - Western blot analysis of various lysates, using Nesprin-3 Rabbit mAb at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for Nesprin-3 Antibody (5P10J3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nesprin-3

Alternate Names

chromosome 14 open reading frame 49, FLJ25605, MGC75267, nesprin-3, NET53

Gene Symbol

SYNE3

Additional Nesprin-3 Products

Product Documents for Nesprin-3 Antibody (5P10J3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nesprin-3 Antibody (5P10J3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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