Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44353

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL11865

Format

Azide and BSA Free
View all Neurofibromin 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P21359, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Neurofibromin 1 Antibody [NBP3-44353]

Immunocytochemistry/ Immunofluorescence: Neurofibromin 1 Antibody [NBP3-44353]

Staining of U-2 OS cells using the Anti-Neurofibromin 1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.

Applications for Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neurofibromin 1

Neurofibromin 1 (NF1) is a suspected negative regulator of the ras signal transduction pathway. Neurofibromin is known to regulate the GTPase HRAS, causing the hydrolyzation and inactivation of GTP. Defects in NF1 lead to Neurofibromatosis disease, Recklinghausen disease or Watson disease. Neurofibromin has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence.

Alternate Names

DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS

Gene Symbol

NF1

Additional Neurofibromin 1 Products

Product Documents for Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Neurofibromin 1 Antibody (CL11865) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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