Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

Novus Biologicals | Catalog # NB100-64392

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ELA10-101.5 (39A)

Format

BSA Free
View all Neutrophil Elastase/ELA2 Primary Antibodies »

Product Specifications

Immunogen

Elastase from human neutrophils

Localization

Secreted

Specificity

NB100-64392 is specific for human neutrophil elastase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) - BSA Free [NB100-64392]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) - BSA Free [NB100-64392]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.

Applications for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neutrophil Elastase/ELA2

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Entrez Gene IDs

1991 (Human)

Gene Symbol

ELANE

UniProt

P08246

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

Certificate of Analysis

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Product Specific Notices for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

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Protocols

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FAQs

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