Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NB100-64392AF488

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # ELA10-101.5 (39A)
View all Neutrophil Elastase/ELA2 Primary Antibodies »

Product Specifications

Immunogen

Elastase from human neutrophils

Reactivity Notes

Not yet tested in other species.

Localization

Secreted

Specificity

NB100-64392 is specific for human neutrophil elastase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [Alexa Fluor® 488] [NB100-64392AF488] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-64392AF488 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50 mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Neutrophil Elastase/ELA2

The ELANE gene (commonly known as neutrophil elastase) encodes a 267 amino acid long, 28 kDA neutrophil elastase protein that functions in regulation of natural killer cells, monocytes, and graulocytes. ELANE is involved in activation of proMMP8, cell adhesion cell-matrix glycoconjugates, selected targets of C/EBPbeta, degradation of the extracellular matrix and collagen, and amb2 integrin signaling. It interacts with various genes GZMB, LRP1, SERPINF2, SERPING1, and SERPINA1. Elane has been linked to cystic fibrosis, asthma, pneumonia, alzheimer's disease, neutropenia, cutis laxia, leg ulcers, wegener's granulomatosis, bronchitis, adult respiratory distress syndrome, and vascular diseases.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (1)

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  • Neutrophil Elastase/ELA2 Antibody (39A) [Alexa Fluor® 488]
    Name: Vincent Giacalone
    Application: Flow Cytometry
    Sample Tested: BAL fluid
    Species: Human
    Verified Customer | Posted 05/27/2020
    Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells
    I used this antibody to stain for surface-bound neutrophil elastase. This enzyme is released from neutrophils following exocytosis of the primary granules and can be captured on the surface of neutrophils and macrophages, but less so on lymphocytes. I purified cells from the BAL of cystic fibrosis patients and stained for analysis by flow cytometry. Macrophages were found to have large amounts of surface-bound elastase, while it is minimal on the T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations.
    Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) [Alexa Fluor® 488] NB100-64392AF488

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