NF-H Antibody (9B12) - BSA Free

Immunocytochemistry/ Immunofluorescence: NF-H Antibody (9B12) - BSA Free [NBP1-05210] -

Wallerian degeneration and mitochondrial degeneration in axon for rat’s distal nerve stump. a, Representative images of toluidine blue staining, immunohistochemistry (NF-H), electron microscopy, before (pre) and 1, 2, and 14 d after axotomy. Immunohistochemistry was obtained the same results for three individuals. Scale bar, * 10 μm, ** 2 μm. Arrows, myelinated axon; arrowheads, demyelinated Schwann cell. b, Quantification of the myelinated axon. c, d, Quantification of the G-ratio [c: cumulative G-ratio per individual, d: scatter plot, Spearman’s rank correlation coefficient and p values showing G-ratio of individual myelinated axons against axon diameter (n = 400)]. The histologic WD was observed significantly from 2 d after axotomy. In axons, before (pre) and 1 and 2 d after axotomy were evaluated, except for after 14 d with completely collapsed axon morphology (n = 4 rat per group). e, Representative images of mitochondrial findings, before (pre) and 1, 2, and 14 d after axotomy. Evaluation was performed in axons and Schwann cells separately. Scale bar, * 200 nm. f, Violin plot of mitochondrial diameter (nm; n = 200). Cumulative mitochondria diameter per individual are also shown (n = 4 rat per group). g, Quantitative analysis of atypical mitochondria (%; n = 4 rat per group). In axons, mitochondria were significantly degenerated from 1 d after axotomy, whereas in Schwann cells, mitochondria were not significantly degenerated until 14 d after axotomy. All histologic evaluations were performed 3 mm distal to the sectional end and corresponding uninjured nerve. Error bars indicate SD; *p < 0.05, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test (for comparison of axons and Schwann cells, respectively). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NF-H Antibody (9B12) - BSA Free [NBP1-05210] -

The gradual decrease of mice longitudinal nerve cross section ATP levels in the distal nerve stump, corresponds to histology. a, Representative ATP images in the longitudinal section before (pre) and 1, 2, and 7 d after axotomy. Scale bar, * 1 mm. P, proximal. D, distal. b, The change of ATP levels with measured ROIs (n = 6 mice per group). Distal nerve stump ATP levels were significantly decreased from 1 d after axotomy. Error bars indicate SD; **p < 0.01, ***p < 0.001, one-way ANOVA followed by the Tukey’s post hoc test. c, Representative enlarged images of two-photon microscopy, immunohistochemistry (left, NF-H/S-100B/DAPI; right, CD68/S-100B/DAPI), and toluidine blue staining, before (pre) and 1, 2, and 7 d after axotomy. Scale bar, * 10 μm. Arrows, macrophage; arrowheads, Schwann cell; double arrows, axonal soma. The ROI for measuring ATP levels was set at 3 mm distal from the sectional end, the center of the longitudinal cross-section, with a 300 × 300-μm square (Extended Data Fig. 3-1). The OFP/GFP ratios ranged from 1.5 to 3.0. All histologic evaluations were performed with the same results for three individuals, 3 mm distal from the sectional end, and the corresponding uninjured nerve. ROI, region of interest. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36894321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.