NGLY1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83792

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NGLY1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEKLLETRCGR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGLY1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human lymph node shows moderate to strong granular cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human prostate shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792]

Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for NGLY1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NGLY1

NGLY1 is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome (Zhou et al., 2006).

Alternate Names

EC 3.5.1.52, hPNGase, N-glycanase 1FLJ12409, Peptide:N-glycanase, peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, PNG1FLJ11005, PNGase

Gene Symbol

NGLY1

Additional NGLY1 Products

Product Documents for NGLY1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NGLY1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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