NHERF-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84944

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NHERF-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQLTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPRLCHLRKGPQGYGFNLH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHERF-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining in human spleen and tonsil tissues using anti-SLC9A3R2 antibody. Corresponding SLC9A3R2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining of human spleen shows high expression.
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining of human tonsil shows low expression as expected.

Applications for NHERF-2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHERF-2

NHERF-2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.

Alternate Names

E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF2MGC104639, NHERF-2NHE3 kinase A regulatory protein E3KARP, OCTS2, SIP-1SRY-interacting protein 1, sodium/hydrogen exchanger, Sodium-hydrogen exchanger regulatory factor 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2, Solute carrier family 9 isoform A3 regulatory factor 2, TKA-1SIP1, Tyrosine kinase activator protein 1

Entrez Gene IDs

9351 (Human)

Gene Symbol

SLC9A3R2

UniProt

Q15599 (Human)

Additional NHERF-2 Products

Product Documents for NHERF-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NHERF-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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