Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

Novus Biologicals | Catalog # NBP2-61674

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7B4H1

Format

BSA Free
View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 4/CHRNA4 (AA: extra 29-242) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1)BSA Free [NBP2-61674]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1)BSA Free [NBP2-61674]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against HEK293 (1) and CHRNA4 (AA: 29-242)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free [NBP2-61674]

Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free [NBP2-61674]

Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mouse mAb against SH-SY5Y (1) cell lysate.
Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1)BSA Free [NBP2-61674]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1)BSA Free [NBP2-61674]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against human CHRNA4 (AA: 29-242) recombinant protein. (Expected MW is 52.5 kDa)
ELISA: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free [NBP2-61674]

ELISA: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free [NBP2-61674]

ELISA: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants.

Long Name

Nicotinic Acetylcholine Receptor alpha 4

Alternate Names

BFNC, CHRNA4, EBN1, ENFL1, nAChR alpha 4, nAchRa4, NACRA4, Nicotinic Acetylcholine Ra4

Gene Symbol

CHRNA4

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) - BSA Free

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  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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