Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

A: A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Novus Biologicals | Catalog # NBP1-00143

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Citations (1)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1.

Epitope

internal region

Specificity

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143] - Analysis of Nicotinic Acetylcholine Receptor alpha 4 in Rat Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml

Application Notes

IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

Long Name

Nicotinic Acetylcholine Receptor alpha 4

Alternate Names

BFNC, CHRNA4, EBN1, ENFL1, nAChR alpha 4, nAchRa4, NACRA4, Nicotinic Acetylcholine Ra4

Entrez Gene IDs

1137 (Human); 11438 (Mouse); 25590 (Rat)

Gene Symbol

CHRNA4

UniProt

NP_000735.1 (Human)

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

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Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neurotransmitter Receptors, Transporters, and Ion Channels

The Cholinergic System in Alzheimer's Disease

Citations for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

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