Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Novus Biologicals | Catalog # NBP2-61677

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D2D10

Format

BSA Free
View all Nicotinic Acetylcholine R alpha 5/CHRNA5 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 5/CHRNA5 (AA: extra 23-254) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against HEK293 (1) and CHRNA5 (AA: 23-254)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free [NBP2-61677]

Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free [NBP2-61677]

Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10)BSA Free [NBP2-61677]

Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate.
ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free [NBP2-61677]

ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free [NBP2-61677]

ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 5/CHRNA5

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).

Long Name

Nicotinic Acetylcholine Receptor alpha 5

Alternate Names

CHRNA5, NACHRA5, Nicotinic Acetylcholine Ra5

Gene Symbol

CHRNA5

Additional Nicotinic Acetylcholine R alpha 5/CHRNA5 Products

Product Documents for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) - BSA Free

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  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A: A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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