Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

Novus Biologicals | Catalog # NBP2-61738

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7F10F1

Format

BSA Free
View all Nicotinic Acetylcholine R alpha 7/CHRNA7 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 7/CHRNA7 (AA: extra 52-259) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) [NBP2-61738] - Analysis using CHRNA7 mAb against HEK293 (1) and CHRNA7 (AA: extra 52-259)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free [NBP2-61738]

Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free [NBP2-61738]

Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) [NBP2-61738] - Analysis of SH-SY5Y cells using CHRNA7 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) [NBP2-61738] - Analysis using CHRNA7 mAb against human CHRNA7 (AA: extra 52-259) recombinant protein. (Expected MW is 50.4 kDa)
Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1)BSA Free [NBP2-61738]

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) [NBP2-61738] - Analysis using CHRNA7 mouse mAb against membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), C6 (3), and HepG2 (4) cell lysate.
ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free [NBP2-61738]

ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free [NBP2-61738]

ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) [NBP2-61738] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 7/CHRNA7

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants.

Long Name

Nicotinic Acetylcholine Receptor alpha 7

Alternate Names

CHRNA7, CHRNA7-2, Nicotinic Acetylcholine Ra 7

Gene Symbol

CHRNA7

Additional Nicotinic Acetylcholine R alpha 7/CHRNA7 Products

Product Documents for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10F1) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Showing  1 - 2 of 2 FAQs Showing All
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