Niemann-Pick type C2 Antibody (S09-8D3)
Novus Biologicals | Catalog # NBP3-14872
Recombinant Monoclonal Antibody
![Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]](https://resources.rndsystems.com/images/products/Niemann-Pick-type-C2-Antibody-S09-8D3-Western-Blot-NBP3-14872-img0001.jpg "Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S09-8D3 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein of human Niemann-Pick type C2 (Uniprot#: P61916)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Niemann-Pick type C2 Antibody (S09-8D3)
Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]
Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872] - Western blot detection of Niemann-Pick type C2 in C6, 3T3, Hela cell lysates using Niemann-Pick type C2 Rabbit mAb (1:1000 diluted). Predicted band size: 17kDa. Observed band size: 17kDa.![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]](https://resources.rndsystems.com/images/products/Niemann-Pick-type-C2-Antibody-S09-8D3-Immunohistochemistry-Paraffin-NBP3-14872-img0002.jpg "Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]")
Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872]
Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872] - Immunohistochemistry of Niemann-Pick type C2 in paraffin-embedded Human lung cancer tissue using Niemann-Pick type C2 Rabbit mAb at dilution 1/50Applications for Niemann-Pick type C2 Antibody (S09-8D3)
Application
Recommended Usage
Immunohistochemistry
1/500
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Niemann-Pick Type C2/NPC2
Alternate Names
EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2
Gene Symbol
NPC2
Additional Niemann-Pick Type C2/NPC2 Products
Product Documents for Niemann-Pick type C2 Antibody (S09-8D3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Niemann-Pick type C2 Antibody (S09-8D3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Niemann-Pick type C2 Antibody (S09-8D3)
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Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
A:
Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.
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