NIFK Antibody (CL2240) - BSA Free

Novus Biologicals | Catalog # NBP2-36749

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2240

Format

BSA Free
View all NIFK Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9BYG3, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NIFK Antibody (CL2240) - BSA Free

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in U251 cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human kidney shows strong nucleolar positivity in renal tubules.
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in HeLa cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human cell line A431 showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in MCF7 cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in U2OS cell line with Anti-MKI67IP monoclonal antibody, showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cells.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human cervix shows strong nucleolar positivity in the epithelial cells.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human endometrium shows nucleolar immunoreactivity in glandular cells.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human prostate shows strong nucleolar positivity in glandular cells.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human rectum shows nucleolar immunoreactivity in glandular and lamina propria cells.

Applications for NIFK Antibody (CL2240) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIFK

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Alternate Names

hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67

Gene Symbol

NIFK

UniProt

Q9BYG3 (Human)

Additional NIFK Products

Product Documents for NIFK Antibody (CL2240) - BSA Free

Certificate of Analysis

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Product Specific Notices for NIFK Antibody (CL2240) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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