NISCH Antibody (2C8) - Azide and BSA Free

Novus Biologicals | Catalog # H00011188-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C8

Format

Azide and BSA Free
View all NISCH Primary Antibodies »

Product Specifications

Immunogen

NISCH (AAH56900, 1246 a.a. ~ 1345 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPAL

Reactivity Notes

Human. Other species not tested.

Specificity

NISCH - nischarin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NISCH Antibody (2C8) - Azide and BSA Free

Sandwich ELISA: NISCH Antibody (2C8) [H00011188-M02] - Detection limit for recombinant GST tagged NISCH is approximately 0.3ng/ml as a capture antibody.

Applications for NISCH Antibody (2C8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NISCH

Mouse Nischarin (NISCH) has been shown to interact with the alpha-5 subunit of integrin and inhibit cell migration. Its human homologue (imidazoline receptor antisera-selected (IRAS)), contains an NH2-terminal extension and is a larger protein of 1504 amino acids consisting of an NH2-terminal PX domain, 5 putative leucine-rich repeats, a predicted coiled-coil domain, and a long COOH-terminal region. It has the ability to homo-oligomerize via its coiled-coil region. The PX domain of IRAS is essential for association with phosphatidylinositol 3-phosphate-enriched endosomal membranes. NISCH may serve as a functional imidazoline I1-receptor.

Long Name

Nischarin

Alternate Names

hIRAS, I-1, I1R, IR1, IRAS, KIAA0975

Entrez Gene IDs

11188 (Human)

Gene Symbol

NISCH

UniProt

AAH56900 (Human)

Additional NISCH Products

Product Documents for NISCH Antibody (2C8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NISCH Antibody (2C8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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