NMT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32168

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NMT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 68 to 118 of human N-Myristoyltransferase 1 using the numbering given in entry NP_066565.1 (GeneID 4836).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NMT1 Antibody - BSA Free

Western Blot: NMT1 Antibody [NBP2-32168]

Western Blot: NMT1 Antibody [NBP2-32168]

Western Blot: NMT1 Antibody [NBP2-32168] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: NMT1 Antibody [NBP2-32168]

Immunoprecipitation: NMT1 Antibody [NBP2-32168]

Immunoprecipitation: NMT1 Antibody [NBP2-32168] - Detection of human NMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for IP at 6 ug per reaction. NMT1 was also immunoprecipitated by rabbit anti-NMT1 antibody NBP2-32167. For blotting immunoprecipitated NMT1, NBP2-32168 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Knockdown Validated: NMT1 Antibody [NBP2-32168]

Western Blot: NMT1 Antibody [NBP2-32168]

NMT1-Antibody-Knockdown-Validated-NBP2-32168-img0004.jpg

Applications for NMT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific publication (PMID: 32686708)

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NMT1

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM)

Alternate Names

alternative, short form NMT-S, glycylpeptide N-tetradecanoyltransferase 1, long form, NMT-L, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, NMTEC 2.3.1.97, N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase

Gene Symbol

NMT1

UniProt

NP_066565.1 (Human)

Additional NMT1 Products

Product Documents for NMT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NMT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMT1 Antibody - BSA Free

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Protocols

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