NO66 Antibody

Novus Biologicals | Catalog # NB100-93381

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NO66 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GAQHSDSKDPR corresponding to internal region according to NP_078920.2.

Epitope

C-GAQHSDSKDPR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NO66 Antibody

Western Blot: NO66 Antibody [NB100-93381]

Western Blot: NO66 Antibody [NB100-93381]

Western Blot: NO66 Antibody [NB100-93381] - Staining of A431 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NO66 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in A431 lysates (calculated MW of 71.1 kDa band according to NP_078920.2). An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NO66

NO66 may play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region

Alternate Names

chromosome 14 open reading frame 169, EC 1.14.11.27, FLJ21802, hsNO66, MAPJD, MYC-associated protein with JmjC domain, NO66lysine-specific demethylase NO66, Nucleolar protein 66

Entrez Gene IDs

79697 (Human); 71952 (Mouse)

Gene Symbol

RIOX1

UniProt

NP_078920.2 (Human)

Additional NO66 Products

Product Documents for NO66 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for NO66 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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