![Western Blot: NOLC1 Antibody [NBP1-22982]](https://resources.rndsystems.com/images/products/NOLC1-Antibody-Western-Blot-NBP1-22982-img0002.jpg "Western Blot: NOLC1 Antibody [NBP1-22982]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 580 and 630 of human nucleolar and coiled-body phosphoprotein 1 using the numbering given in entry NP_004732.2 (GeneID 9221).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOLC1 Antibody
Western Blot: NOLC1 Antibody [NBP1-22982]
Western Blot: NOLC1 Antibody [NBP1-22982] - Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti- NOPP140 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody NBP1-22981, which recognizes an upstream epitope.Applications for NOLC1 Antibody
Application
Recommended Usage
Immunoprecipitation
5-15 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NOLC1
Alternate Names
140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035NS5ATP13, NOPP130, Nopp140, Nucleolar 130 kDa protein, nucleolar and coiled-body phosphoprotein 1, nucleolar and coiled-body phosphprotein 1, Nucleolar phosphoprotein p130, nucleolar protein p130, P130
Entrez Gene IDs
9221 (Human)
Gene Symbol
NOLC1
UniProt
Additional NOLC1 Products
Product Documents for NOLC1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NOLC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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