NOX5 Antibody

Novus Biologicals | Catalog # NBP1-68862

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NOX5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EWHPFTISSAPEQKD corresponding to internal region according to NP_078781.3, NP_001171708.1, NP_001171709.1.

Epitope

C-EWHPFTISSAPEQKD

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32165681).

Specificity

This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NOX5 Antibody

Western Blot: NOX5 Antibody [NBP1-68862]

Western Blot: NOX5 Antibody [NBP1-68862]

Western Blot: NOX5 Antibody [NBP1-68862] - NBP1-68862 (0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NOX5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human testis lysates (calculated MW of 86.4 kDa band according to NP_078781.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NOX5

NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependentmanner.(supplied by OMIM)

Long Name

NADPH oxidase 5

Alternate Names

EC 1.6.3.-, MGC149776, MGC149777, NADPH oxidase 5, NADPH oxidase, EF-hand calcium binding domain 5, NOX5A, NOX5B

Entrez Gene IDs

79400 (Human)

Gene Symbol

NOX5

UniProt

NP_078781.3 (Human)

Additional NOX5 Products

Product Documents for NOX5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NOX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NOX5 Antibody

There are currently no reviews for this product. Be the first to review NOX5 Antibody and earn rewards!

Have you used NOX5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies