NPY2R Antibody

Novus Biologicals | Catalog # NB100-57847

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Canine (100%), Human (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NPY2R Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KAKKNLEVKKNN corresponding to internal region according to NP_032757.1.

Epitope

KAKKNLEVKKNN

Specificity

Please note this antibody was designed using the Mouse sequence NP_032757.1, which differs by 1 amino acid from the Human sequence NP_000901.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NPY2R Antibody

Western Blot: NPY2R Antibody [NB100-57847]

Western Blot: NPY2R Antibody [NB100-57847]

Western Blot: NPY2R Antibody [NB100-57847] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NPY2R Antibody

Application
Recommended Usage

Immunoprecipitation

1:50

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
A customer has resported successful usage for IP of human Y2R, but the Human Y2R could not be detected in WB by this antibody. WB: Approx. 40-45 kDa band observed in rat brain lysates (calculated MW of 42.6 kDa band according to NP_032757.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NPY2R

NPY2R is widely expressed in the central nervous system, with particularly high abundance in the hypothalamus. The hypothalamus is known to be important for appetite regulation and thus variants in this gene could be associated with obesity.

Long Name

Neuropeptide Y Receptor Y2

Alternate Names

Y2 Receptor

Entrez Gene IDs

4887 (Human); 18167 (Mouse); 66024 (Rat)

Gene Symbol

NPY2R

UniProt

NP_032757.1 (Human)

Additional NPY2R Products

Product Documents for NPY2R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NPY2R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for NPY2R Antibody

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  • Q: Which cell lysate should I use with Anti- human Neuropeptide Y2R (cat# NB100-57847)?

    A: NPY2R: High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.

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