Nrf1 Antibody (4Q4E7)

Novus Biologicals | Catalog # NBP3-16161

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Q4E7 expressed in HEK293
View all Nrf1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF1 (Q16656). LVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nrf1 Antibody (4Q4E7)

Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Immunohistochemistry of paraffin-embedded human colon using Nrf1 Rabbit mAb (NBP3-16161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161]

Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Nrf1 Antibody (4Q4E7)

Chromatin Immunoprecipitation: Nrf1 Antibody (4Q4E7) [NBP3-16161] -

Chromatin Immunoprecipitation: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Nrf1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Nrf1 Antibody (4Q4E7)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5μg antibody for 10μg-15μg of Chromatin

Chromatin Immunoprecipitation Sequencing

10⁵ cells /1 μg

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nrf1

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Gene Symbol

NRF1

Additional Nrf1 Products

Product Documents for Nrf1 Antibody (4Q4E7)

Certificate of Analysis

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Product Specific Notices for Nrf1 Antibody (4Q4E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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