NRSF Antibody (CL0381) - BSA Free

Novus Biologicals | Catalog # NBP2-42048

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0381

Format

BSA Free
View all REST/NRSF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13127, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NRSF Antibody (CL0381) - BSA Free

Immunocytochemistry/ Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048]

Immunocytochemistry/ Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048]

Immunocytochemistry/Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048] - Staining of U-2 OS cells using the Anti-REST monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human cerebral cortex shows no nuclear positivity in neuronal cells as expected.
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.

Applications for NRSF Antibody (CL0381) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: REST/NRSF

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Long Name

RE1-Silencing Transcription Factor

Alternate Names

NRSF, XBR

Gene Symbol

REST

Additional REST/NRSF Products

Product Documents for NRSF Antibody (CL0381) - BSA Free

Certificate of Analysis

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Product Specific Notices for NRSF Antibody (CL0381) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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