NSFL1C Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-36529

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NSFL1C Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NSFL1C Antibody - BSA Free

Western Blot: NSFL1C Antibody [NBP2-36529]

Western Blot: NSFL1C Antibody [NBP2-36529]

Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NSFL1C Antibody [NBP2-36529]

Immunoprecipitation: NSFL1C Antibody [NBP2-36529]

Immunoprecipitation: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated NSFL1C, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NSFL1C Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NSFL1C

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, p47, p97 cofactor p47, SHP1 homolog, UBX domain protein 2C, UBX domain-containing protein 2C, UBX1, UBXD10, UBXN2CMGC3347

Gene Symbol

NSFL1C

UniProt

NP_057227.2 (Human)

Additional NSFL1C Products

Product Documents for NSFL1C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NSFL1C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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