NT5C2 Antibody

Novus Biologicals | Catalog # NBP1-85526

Novus Biologicals
Discontinued Product
NBP1-85526 has been discontinued. View all NT5C2 products.

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NT5C2 Antibody

Western Blot: 0 [NBP1-85526]

Western Blot: 0 [NBP1-85526]

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.
Western Blot: 0 [NBP1-85526]

Western Blot: 0 [NBP1-85526]

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in control (vector only transfected HEK293T lysate) and NT5C2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: 0 [NBP1-85526]

Western Blot: 0 [NBP1-85526]

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in human cell line A-431 and human cell line HeLa.
Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human testis shows cytoplasmic positivity in seminiferus duct cells.
Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human liver shows weak to moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526]

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.

Applications for NT5C2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NT5C2

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Alternate Names

5'-nucleotidase (purine), cytosolic type B, 5'-nucleotidase, cytosolic II, cN-II, Cytosolic 5'-nucleotidase II, EC 3.1.3, EC 3.1.3.5, GMP, NT5BIMP-specific 5'-NT, NT5CP, PNT5cytosolic purine 5'-nucleotidase, purine 5' nucleotidase

Gene Symbol

NT5C2

Additional NT5C2 Products

Product Documents for NT5C2 Antibody

Certificate of Analysis

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Product Specific Notices for NT5C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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