NTH1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-302

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NTH1 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human NTH1. [UniProt# P78549]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NTH1 Antibody - BSA Free

Western Blot: NTH1 Antibody [NB100-302]

Western Blot: NTH1 Antibody [NB100-302]

Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.
Immunocytochemistry/ Immunofluorescence: NTH1 Antibody [NB100-302]

Immunocytochemistry/ Immunofluorescence: NTH1 Antibody [NB100-302]

Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Applications for NTH1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000-1:5000
Application Notes
In Western blot, a band is seen at ~37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NTH1

NTH1 (endonuclease III-like protein 1 or NTHL1) has both apurinic/apyrimidinic endonuclease activity and a DNA N-glycosylase activity. The C-O-P bond 3' to apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. NTH1 associates with 1 4Fe-4S cluster and this cluster has been proposed to involve in proper positioning of enzyme along the DNA strand. With potential for incising damaged DNA at cytosines, thymines and guanines, NTH1 acts on a damaged strand 5' from the damaged site, for playing essential role in repair of both oxidative DNA damage and spontaneous mutagenic lesions. Localized in the nucleus, NTH1 is widely expressed with highest levels in heart and its expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.

Long Name

Nth Endonuclease III-like 1

Alternate Names

NTHL1, OCTS3

Entrez Gene IDs

4913 (Human)

Gene Symbol

NTHL1

UniProt

P78549

Additional NTH1 Products

Product Documents for NTH1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NTH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

Citations for NTH1 Antibody - BSA Free

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Protocols

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FAQs for NTH1 Antibody - BSA Free

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  • Q: How much immunogen in mass did you use to get this result? What's your secondary detection approach?

    A:

    Please see our full Western blot protocol at this link. NB7183 is the secondary antibody we use and we loaded 40 ug of protein in the gel.

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