NTS1/NTSR1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS938

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NTS1/NTSR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 17 amino acid peptide from 3rd cytoplasmic domain of human NTS1/NTSR1.

Epitope

3rd cytoplasmic domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (88%), Canine (88%), Equine (88%).

Specificity

Human NTSR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for NTS1/NTSR1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: Neurotensin Receptor 1 Antibody [NLS938] - Human Bladder, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: Neurotensin Receptor 1 Antibody [NLS938] - Analysis of anti-NTSR1 / NTR antibody with brain, neurons and glia at 16 ug/ ml.
Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: Neurotensin Receptor 1 Antibody [NLS938] - Analysis of anti-NTSR1 / NTR antibody with human adrenal, pheochromocytoma.
Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: Neurotensin Receptor 1 Antibody [NLS938] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: NTS1/NTSR1 Antibody - BSA Free [NLS938]

Immunohistochemistry-Paraffin: Neurotensin Receptor 1 Antibody [NLS938] - Human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for NTS1/NTSR1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

16 ug/ml
Application Notes
.

Reviewed Applications

Read 1 review rated 3 using NLS938 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: NTS1/NTSR1

NTR1, a Neurotensin Receptor, activates a phosphatidylinositol-calcium second messenger system in the central and peripheral nervous systems. It mediates the functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. Neurotensin Receptor 1 expression is enhanced in pancreatic cancer and in chronic pancreatitis (Wang et al, 2000). Two differentially glycosylated forms of the NT1 receptor have been identified in rats, a 54-kDa form that is expressed from birth to adulthood, and a 52-kDa form only detected at 10 and 15 days postnatal. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.

Long Name

Neurotensin Receptor 1

Alternate Names

NTR1, NTRH, NTRR, NTSR1

Entrez Gene IDs

4923 (Human)

Gene Symbol

NTSR1

UniProt

P30989 (Human)

Additional NTS1/NTSR1 Products

Product Documents for NTS1/NTSR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NTS1/NTSR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NTS1/NTSR1 Antibody - BSA Free

Customer Reviews for NTS1/NTSR1 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23901225
    Species: Human
    Verified Customer | Posted 12/29/2014

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Protocols

View specific protocols for NTS1/NTSR1 Antibody - BSA Free (NLS938):

Immunohistochemistry Protocol for Neurotensin Receptor 1 Antibody (NLS938):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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