Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NUCKS1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 147 and 197 of human nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate using the numbering given in entry NP_073568.2 (GeneID 64710).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit NUCKS1 Antibody - BSA Free (NB100-74635) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NUCKS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: NUCKS1 Antibody [NB100-74635]

Immunohistochemistry-Paraffin: NUCKS1 Antibody [NB100-74635]

Immunohistochemistry-Paraffin: NUCKS1 Antibody [NB100-74635] - Detection of human NUCKS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUCKS (NB100-74635). Detection: DAB
Immunoprecipitation: NUCKS1 Antibody [NB100-74635]

Immunoprecipitation: NUCKS1 Antibody [NB100-74635]

Immunoprecipitation: NUCKS1 Antibody [NB100-74635] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: NUCKS1 antibody NB100-74635 used for IP at 3 ug per reaction. NUCKS was also immunoprecipitated by NUCKS1 antibody NB100-74634. For blotting immunoprecipitated NUCKS, NB100-74635 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NUCKS1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 -1:5000

Immunohistochemistry-Paraffin

1:1000 -1:5000

Immunoprecipitation

2 - 5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NUCKS1

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.

Alternate Names

FLJ21480, FLJ32016, FLJ38536, NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate, nuclear casein kinase and cyclin-dependent kinase substrate 1, nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate, P1, potential LAG1 interactor

Entrez Gene IDs

64710 (Human)

Gene Symbol

NUCKS1

UniProt

NP_073568.2 (Human)

Additional NUCKS1 Products

Product Documents for NUCKS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NUCKS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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