Nuclear Antigen Antibody (235-1)

Novus Biologicals | Catalog # NBP2-34342

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Primate

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 235-1
View all Nuclear Antigen Primary Antibodies »

Product Specifications

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:35327471).Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Marker

Human Cell Marker

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34525)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nuclear Antigen Antibody (235-1)

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Immunofluorescent staining of PFA-fixed MCF-7 cells with Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).
Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Acetone-fixed frozen human Tonsil stained with Nuclear Antigen Antibody using HRP-DAB detection.
Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Flow Cytometric analysis of Human Nuclear Antigen on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Nuclear Antigen Monoclonal Antibody (235-1).
Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Nuclear Antigen Antibody followed by goat anti-Mouse IgG-CF488 (Green). Counter stained with Phalloidin (red).
Flow (Intracellular): Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow (Intracellular): Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow (Intracellular): Nuclear Antigen Antibody (235-1) [NBP2-34342] - An intracellular stain was performed on HeLa cells with Human NBP2-34525AF647 (blue) along with a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes. Both antibodies were conjugated to Alexa Fluor 647. A negative control, RAW 264.7 cells, was also stained to ensure antibody specificity (B). Image from the Alexa Fluor 647 version of this antibody.
Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342]

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with Nuclear Antigen Antibody followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Nuclear Antigen Antibody (235-1)

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342] -

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Immunofluorescent staining of PFA-fixed K562 cells with Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).
Nuclear Antigen Antibody (235-1)

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] -

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Flow Cytometric analysis of Human Nuclear Antigen on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Nuclear Antigen Monoclonal Antibody (235-1).
Nuclear Antigen Antibody (235-1)

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] -

Flow Cytometry: Nuclear Antigen Antibody (235-1) [NBP2-34342] - Flow Cytometric Analysis of PFA-fixed K562 cells with Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for Nuclear Antigen Antibody (235-1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1000-2000 ug/500 ug protein lysate
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (235-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nuclear Antigen Antibody (235-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nuclear Antigen Antibody (235-1)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nuclear Antigen Antibody (235-1)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Is this antibody good for Immunofluorescence in formalin fixed tissues? 

    A: This product has been tested in both ICC and IHC with frozen tissues. For both the ICC and IHC protocol, our lab uses 4% paraformaldehyde fixation. So this product should work fine with formalin fixation methods as well.

  • Q: Please help us to confirm the Molecular Weight of Human Nuclear Antigen（NBP2-34342)

    A: NBP2-34342 is a human nuclear antigen antibody in which nuclei of human myeloid leukemia biopsy cells were used for immunization. This antibody has not been validated for WB, so this will not be guaranteed, however the Molecular Weight of Antigen is described as: 70kDa & 80kDa.

  • Q: Is this antibody good for Immunofluorescence in formalin fixed tissues? 

    A: This product has been tested in both ICC and IHC with frozen tissues. For both the ICC and IHC protocol, our lab uses 4% paraformaldehyde fixation. So this product should work fine with formalin fixation methods as well.

  • Q: Please help us to confirm the Molecular Weight of Human Nuclear Antigen（NBP2-34342)

    A: NBP2-34342 is a human nuclear antigen antibody in which nuclei of human myeloid leukemia biopsy cells were used for immunization. This antibody has not been validated for WB, so this will not be guaranteed, however the Molecular Weight of Antigen is described as: 70kDa & 80kDa.

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