Nuclear Antigen Antibody (235-1R)

Novus Biologicals | Catalog # NBP3-13912

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 235-1R
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Product Specifications

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Reactivity Notes

Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Marker

Human Cell Marker

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

70,80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14102)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nuclear Antigen Antibody (235-1R)

Nuclear Antigen Antibody (235-1R)

Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).

Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).
Nuclear Antigen Antibody (235-1R)

Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).

Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).

Applications for Nuclear Antigen Antibody (235-1R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

1-2 ug/ml

Immunoprecipitation

1000-2000 ug/500 ug protein lysate
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.

Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT

Immunoprecipitation: 1-2 g/500ug protein lysate)
Immunoprecipitation: 1-2 gram/500 ug protein lysate

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (235-1R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nuclear Antigen Antibody (235-1R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nuclear Antigen Antibody (235-1R)

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Protocols

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