Nuclear Antigen Antibody (NM106) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48213

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NM106

Format

IHC-Prediluted
View all Nuclear Antigen Primary Antibodies »

Product Specifications

Immunogen

Nuclei of HL60 cells

Reactivity Notes

Does not react with Porcine.

Localization

Nuclei

Marker

Pan-Nuclear Marker

Specificity

This monoclonal antibody is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Nuclear Antigen Antibody (NM106) - IHC-Prediluted

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded Rat Lung stained with Pan Nuclear Antigen Antibody.
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pan Nuclear Antigen Antibody.
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pan Nuclear Antigen Antibody.
Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213]

Immunohistochemistry-Paraffin: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] - Formalin-fixed, paraffin-embedded Rat Colon stained with Pan Nuclear Antigen Antibody.
Nuclear Antigen Antibody (NM106) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] -

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (NM106) - IHC-Prediluted [NBP2-48213] -

Immunofluorescentstaining of PFA-fixedHeLa cells with Nuclear Antigen Antibody (NM106) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).

Applications for Nuclear Antigen Antibody (NM106) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunocytochemistry Acetone-fixed cells 0.25 - 0.5 ug/ml for 30 minutes at RT; Immunohistology Formalin-paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (NM106) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Nuclear Antigen Antibody (NM106) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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