NUSAP1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # H00051203-B01P
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Scientific Data Images for NUSAP1 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: NUSAP1 Antibody [H00051203-B01P]
Immunocytochemistry/Immunofluorescence: NUSAP1 Antibody [H00051203-B01P] - Analysis of purified antibody to NUSAP1 on HeLa cell. (antibody concentration 10 ug/ml)Western Blot: NUSAP1 Antibody [H00051203-B01P]
Western Blot: NUSAP1 Antibody [H00051203-B01P] - Analysis of NUSAP1 expression in transfected 293T cell line by NUSAP1 polyclonal antibody. Lane 1: NUSAP1 transfected lysate(24.86 KDa). Lane 2: Non-transfected lysate.Western Blot: NUSAP1 Antibody [H00051203-B01P] -
Western Blot: NUSAP1 Antibody [H00051203-B01P] - Depletion of NUSAP1 induces DNA damage in GBM cells. a Mini ontology obtained in French’s database using gene sets related to NUSAP1, which were identified by a significant difference of P < 0.001. b Tailed DNA in single cells depleted of NUSAP1. c IF staining of gamma -H2AX in NUSAP1-knockdown GBM cells. d Quantification of gamma -H2AX foci in the indicated GBM cells. e Western blot analyses of DDR proteins in GBM with NUSAP1 knockdown. f The level of the indicated proteins in GBM cells with NUSAP1 knockdown for 0 h, 24 h, 48 h, 72 h, & 96 h. All data are used as the mean ± SD, & significant differences were determined by Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. P < 0.05 was considered statistically significant Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32317623), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: NUSAP1 Antibody [H00051203-B01P] -
Western Blot: NUSAP1 Antibody [H00051203-B01P] - Silencing NUSAP1 inhibits cell proliferation & causes apoptosis in GBM cells. a Western blot analyses of NUSAP1 in cells with NUSAP1 knockdown. b The morphology & cell number of GBM cells after knocking down NUSAP1. c Viability of NUSAP1-knockdown GBM cells. d BrdU-positive GBM cells after knocking down NUSAP1. e Flow cytometry analyses of apoptosis in GBM cells with NUSAP1 knockdown. f The expression of the apoptotic proteins bcl2 & cleaved caspase-3 in cells with NUSAP1 knockdown. g Caspase-3/7 activity of GBM cells with NUSAP1 knockdown. All data are expressed as the mean ± SD, & significant differences were determined by Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. P < 0.05 was considered statistically significant Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32317623), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: NUSAP1 Antibody [H00051203-B01P] -
Western Blot: NUSAP1 Antibody [H00051203-B01P] - Depletion of NUSAP1 induces DNA damage in GBM cells. a Mini ontology obtained in French’s database using gene sets related to NUSAP1, which were identified by a significant difference of P < 0.001. b Tailed DNA in single cells depleted of NUSAP1. c IF staining of gamma -H2AX in NUSAP1-knockdown GBM cells. d Quantification of gamma -H2AX foci in the indicated GBM cells. e Western blot analyses of DDR proteins in GBM with NUSAP1 knockdown. f The level of the indicated proteins in GBM cells with NUSAP1 knockdown for 0 h, 24 h, 48 h, 72 h, & 96 h. All data are used as the mean ± SD, & significant differences were determined by Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. P < 0.05 was considered statistically significant Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32317623), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for NUSAP1 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence
Western Blot
Formulation, Preparation, and Storage
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Formulation
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Preservative
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Background: NUSAP1
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional NUSAP1 Products
Product Documents for NUSAP1 Antibody - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for NUSAP1 Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NUSAP1 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars