OBFC1 Antibody (OTI2E4)

Western Blot: OBFC1 Antibody (OTI2E4) [NBP2-01006] -

CST deficiency results in SCC loss.A, Western blot of STN1 knockdown and MAD2 levels in HeLa cells. Actinin was used as the loading control. B, example images of metaphase spreads with normal chromosomes or SCC loss. The scale bar represents 5 μm. C, fold increase in cohesion loss after metaphase spread analysis. n = 3 independent, biological replicates. D, representative images of chromosome FISH from cells isolated by mitotic shake-off. Red represents centromere 6 probe; blue represents DAPI. The scale bar represents 5 μm. E, fold increase in nuclei with >4 chromosome 6 foci. n = 4 independent, biological replicates. F, knockdown of CTC1, STN1, or TEN1 by siRNA in HeLa cells. siNT was used as the nontarget control and actinin as the loading control. G, graph of SCC loss after metaphase spread analysis, as indicated. n = 3 independent, biological replicates. (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). CST, CTC1-STN1-TEN1; SCC, sister chromatid cohesion; shNT, nontargeting shRNA; shSTN1, shRNA knockdown of STN1; shSTN1+Flag-STN1, shSTN1-7 cells plus shRNA-resistant Flag-STN1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34339741), licensed under a CC-BY license. Not internally tested by Novus Biologicals.