Oct-4B Antibody (OTI7H1)

Novus Biologicals | Catalog # NBP2-45376

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7H1
View all Oct-4B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Oct-4B Antibody (OTI7H1)

Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1P1.
Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1P1.
Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]

Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of CRL1973 cells.

Applications for Oct-4B Antibody (OTI7H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200 - 1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Oct-4B

Long Name

Octamer-binding Transcription Factor 4B

Alternate Names

OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1

Gene Symbol

POU5F1

Additional Oct-4B Products

Product Documents for Oct-4B Antibody (OTI7H1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Oct-4B Antibody (OTI7H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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