OGG1 Antibody (4E8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80890

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4E8

Format

Azide and BSA Free
View all OGG1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300) [UniProt# O15527]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for OGG1 Antibody (4E8) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunocytochemistry/Immunofluorescence: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (4E8) at a 1:100 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma. Image from the standard format of this antibody.
Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma. Image from the standard format of this antibody.
Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890]

Immunohistochemistry: OGG1 Antibody (4E8) - Azide and BSA Free [NBP2-80890] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma. Image from the standard format of this antibody.

Applications for OGG1 Antibody (4E8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2-5 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OGG1

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

8-oxoGuanine DNA Glycosylase

Alternate Names

8-hydroxyguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, AP lyase, DNA-apurinic or apyrimidinic site lyase, HOGG1, MMH, MUTMOGH1HMMH, N-glycosylase/DNA lyase

Gene Symbol

OGG1

Additional OGG1 Products

Product Documents for OGG1 Antibody (4E8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for OGG1 Antibody (4E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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