Olig2 Antibody (OLIG2/6695R)

Novus Biologicals | Catalog # NBP3-13830

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # OLIG2/6695R
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa200-300) of human Olig2 protein (exact sequence is proprietary) (Uniprot: Q13516)

Localization

Nucleus, Cytoplasm

Marker

Marker of Glial Brain Tumors

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14217)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Olig2 Antibody (OLIG2/6695R)

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human glioma stained withOlig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human cerebrum stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human oligodendroglioma stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human cerebellum stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830]

Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Stained with Olig2 antibody (OLIG2/6695R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary; secondary antibody negative control.

Applications for Olig2 Antibody (OLIG2/6695R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

UniProt

Q13516

Additional Olig2 Products

Product Documents for Olig2 Antibody (OLIG2/6695R)

Certificate of Analysis

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Product Specific Notices for Olig2 Antibody (OLIG2/6695R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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