Orexin A and orexin B are neuropeptide hormones derived from proteolytic processing of the same orexin precursor protein. Orexins are named for their orexigenic-inducing (orexis; Greek for appetite) activity. They are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. Orexins A and B are less than 50% amino acid identical. Orexin A is a ligand for two 7-transmembrane orexin receptors, OX1R and OX2R, while orexin B preferentially binds OX2R. Defects in the orexin precursor are a cause of narcolepsy in humans.
Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-80234
Loading...
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 ul)
Sensitivity
0.234 ng/mL
Assay Range
0.391-25 ng/mL
Loading...
Product Specifications
Assay Type
Competitive-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Human
Specificity
This kit recognizes Human OXB in samples. No significant cross-reactivity or interference between Human OXB and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 6.32%
Inter-Assay Precision (Precision between assays) CV% 6.65%
Recovery for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
Recovery
87-110%
Linearity
Scientific Data Images for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
ELISA: Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric) [NBP2-80234] -
ELISA: Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric) [NBP2-80234] - Standard Curve ReferenceKit Contents for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100X)
- Concentrated HRP Conjugate (100X)
- Concentrated Wash Buffer (25X)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Orexin B/Hypocretin-2
Additional Orexin B/Hypocretin-2 Products
Product Documents for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Customer Reviews for Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)
There are currently no reviews for this product. Be the first to review Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric) and earn rewards!
Have you used Human Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...