Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

Novus Biologicals | Catalog # H00003062-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E3

Format

Azide and BSA Free
View all Orexin R2/HCRTR2 Primary Antibodies »

Product Specifications

Immunogen

HCRTR2 (NP_001517, 1 a.a. ~ 54 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYE

Localization

Isoform 1 and isoform 4: Cell membrane; single pass type I membrane protein. Isoform 2 and isoform 3: Secreted protein.

Specificity

HCRTR2 - hypocretin (orexin) receptor 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3.Lane 1: HCRTR2 transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

Western Blot: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCRTR2 monoclonal antibody (M01), clone 1E3 (Cat # H00003062-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01]

ELISA: Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Orexin R2/HCRTR2 Antibody (1E3) [H00003062-M01] - Detection limit for recombinant GST tagged HCRTR2 is approximately 0.03ng/ml as a capture antibody.

Applications for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00003062-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Orexin R2/HCRTR2

HCRTR2 is a G-protein coupled receptor expressed exclusively in the brain. It has 64% identity with HCRTR1. HCRTR2 binds both orexin A and orexin B neuropeptides. HCRTR2 is involved in the central feedback mechanism that regulates feeding behaviour.

Long Name

Hypocretin Receptor Type 2

Alternate Names

HCRTR2, Orexin Receptor 2, OrexinR2, OX2R

Entrez Gene IDs

3062 (Human)

Gene Symbol

HCRTR2

OMIM

602393 (Human)

UniProt

NP_001517 (Human)

Additional Orexin R2/HCRTR2 Products

Product Documents for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free

Customer Reviews for Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: H00003062-Q01 and H00003062-B01, Sample Amount:.01,.025,.05, 0.1, 0.2 ug
    Species: Human
    Verified Customer | Posted 06/08/2011
    Orexin R2/HCRTR2 Antibody (1E3) - Azide and BSA Free H00003062-M01

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