Ornithine Carbamoyltransferase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31582

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Primate

Predicted:

Bovine (94%), Porcine (94%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ornithine Carbamoyltransferase/OTC Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ornithine Carbamoyltransferase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ornithine Carbamoyltransferase Antibody - BSA Free

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP1-31582]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP1-31582]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP1-31582] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582]

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582]

Ornithine-Carbamoyltransferase-Antibody-Western-Blot-NBP1-31582-img0008.jpg
Ornithine Carbamoyltransferase Antibody

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] -

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] - OTC enzyme & biochemical parameters in spf-J males.Spf-J & WT were maintained on a regular chow diet (22% protein) & euthanized at 8 weeks. Blood was collected by retro-orbital bleeding with plasma separated & stored at −80°C until use. Tissues were snap frozen & stored at −80°C until use. A) mRNA level in spf-J (N = 4) & WT (N = 4) livers. B) Immunoblot of OTC protein. C) OTC enzyme activity in liver homogenates from spf-j (N = 6) & WT (N = 4). D) Plasma ammonia in spf-j (N = 7) & WT (N = 6). E) Plasma orotate in spf-j (N = 5) & WT (N = 6). Hatched bar indicates P < 0.05. WT – wild-type. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0116594), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
Ornithine Carbamoyltransferase Antibody - BSA Free

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] -

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ornithine Carbamoyltransferase antibody (NBP1-31582) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Ornithine Carbamoyltransferase Antibody - BSA Free

Western Blot: Ornithine Carbamoyltransferase Antibody [NBP1-31582] -

Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ornithine Carbamoyltransferase antibody (NBP1-31582) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Ornithine Carbamoyltransferase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:30000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ornithine Carbamoyltransferase/OTC

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Alternate Names

OCTD, Ornithine Transcarbamylase, OTC, OTCase

Entrez Gene IDs

5009 (Human)

Gene Symbol

OTC

UniProt

P00480

Additional Ornithine Carbamoyltransferase/OTC Products

Product Documents for Ornithine Carbamoyltransferase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ornithine Carbamoyltransferase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Ornithine Carbamoyltransferase Antibody - BSA Free

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Protocols

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