OTP Antibody (CL11225)
Novus Biologicals | Catalog # NBP3-52439
Recombinant Monoclonal Antibody
![OTP Antibody (CL11225) Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-52439]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunocytochemistry-immunofluorescence-639191183778775794.jpg "Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-52439]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL11225
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q5XKR4, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for OTP Antibody (CL11225)
Immunocytochemistry/ Immunofluorescence: OTP Antibody (CL11225) [NBP3-52439]
Staining of MCF7 cells, showing specific staining in nuclear bodies and in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively.![OTP Antibody (CL11225) Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunohistochemistry-paraffin-639191183780494011.jpg "Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP3-52441]")
Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP3-52441]
Staining of human fallopian tube shows no positivity in glandular cells as expected.![OTP Antibody (CL11225) Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0320) [NBP3-52448]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunohistochemistry-paraffin-639191183780998140.jpg "Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0320) [NBP3-52448]")
Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0320) [NBP3-52448]
Staining of human hypothalamus shows strong nuclear positivity in a subset of neurons.![OTP Antibody (CL11225) Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP3-52445]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunohistochemistry-paraffin-639191183781866849.jpg "Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP3-52445]")
Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP3-52445]
Staining of human lung tumor (typical carcinoid) shows strong nuclear positivity in tumor cells.![OTP Antibody (CL11225) Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunohistochemistry-paraffin-639191183784607033.jpg "Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]")
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]
Staining of human tonsil shows no positivity in non-germinal center cells as expected.![OTP Antibody (CL11225) Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]](https://resources.rndsystems.com/images/products/nbp3-52439_-immunohistochemistry-paraffin-639191183783824351.jpg "Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]")
Immunohistochemistry-Paraffin: YAP1 Antibody (CL13628) [NBP3-52440]
Staining of rat hypothalamus shows strong nuclear positivity in a subset of neurons.Applications for OTP Antibody (CL11225)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehaloseilized from a 0.2 um filtered solution in PBS with Trehalose
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: OTP
Alternate Names
homeobox protein orthopedia, MGC3161, orthopedia homeobox, orthopedia homolog, orthopedia homolog (Drosophila)
Gene Symbol
OTP
Additional OTP Products
Product Documents for OTP Antibody (CL11225)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OTP Antibody (CL11225)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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