Otubain-1 Antibody

Western Blot: Otubain-1 Antibody [NBP1-49934]

Western Blot: Otubain-1 Antibody [NBP1-49934] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Western Blot: Otubain-1 Antibody [NBP1-49934] -

OTUB1 inhibits breast cancer growth in vivo. (A) Control, OTUB1−/− and OTUB1−/− + CCN6 4T1 cells were injected into nude mice and tumour volume was measured (n = 6 for all groups) (mean +/- SEM, NC vs. OTUB1−/−: *p <.05, **p <.01; NC vs. OTUB1−/− + CCN6: #p <.05, ##p <.01). On day 9 after tumour inoculation, mice were sacrificed and tumours were collected. Tumour (B) size and (C) weight were measured (n = 6 for all groups) (mean +/- SEM, *p <.05, **p <.01). (D) Whole‐cell lysates of tumour samples were analysed by Western blot for OTUB1, CCN6 and GAPDH. (E) Tumour samples were stained for Ki‐67 and immunoreactivity was detected by 3,3′‐diaminobenzidine chromogen (brown). Sections were counterstained with haematoxylin (blue) (scale bar = 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37608493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Otubain-1 Antibody [NBP1-49934] -

OTUB1 expression is positively correlated with CCN6 in clinical samples. (A) An invasive ductal carcinoma (Grade II, moderately differentiated) sample (T) and the adjacent non‐tumour tissue (N) were analysed with H&E staining and immunohistochemistry for OTUB1 and CCN6 (scale bar = 100 μm). (B) A breast intraductal papilloma sample (T) and the adjacent non‐tumour tissue (N) were analysed with H&E staining and immunohistochemistry for OTUB1 and CCN6 (scale bar = 100 μm). (C) Clinical samples were analysed with Western blot for OTUB1, CCN6 and GAPDH. (D) Correlation between the expression of OTUB1 and CCN6 in tumour tissues (upper panel) and adjacent non‐tumour tissues (lower panel). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37608493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.