Otubain-1 Antibody

Name: Otubain-1 Antibody
Brand: Novus Biologicals
SKU: NBP1-49935
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-49935

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 40 and 80 of human OTU Domain, Ubiquitin Aldehyde Binding 1 using the numbering given in entry NP_060140.2 (GeneID 55611).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otubain-1 Antibody

Western Blot: Otubain-1 Antibody [NBP1-49935]

Western Blot: Otubain-1 Antibody [NBP1-49935] - OTUB1 Antibody [NBP1-49935] - Detection of Human and Mouse OTUB1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-OTUB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. OTUB1 was also immunoprecipitated by rabbit anti-OTUB1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated OTUB1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for Otubain-1 Antibody

Application

Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Gene Symbol

OTUB1

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Product Documents for Otubain-1 Antibody

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Certificate of Analysis

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Product Specific Notices for Otubain-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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