Otx2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86533

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otx2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533]

Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.
Otx2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Otx2 Antibody - BSA Free [NBP1-86533]

Chromatin Immunoprecipitation-exo-Seq: Otx2 Antibody - BSA Free [NBP1-86533]

ChIP-Exo-Seq composite graph for Anti-OTX2 (NBP1-86533) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
Otx2 Antibody - BSA Free

Otx2 Antibody [NBP1-86533] -

Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Otx2 Antibody - BSA Free

Otx2 Antibody [NBP1-86533] -

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Otx2 Antibody - BSA Free

Otx2 Antibody [NBP1-86533] -

Staining of human liver shows no positivity in hepatocytes as expected.

Applications for Otx2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

Additional Otx2 Products

Product Documents for Otx2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Otx2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Otx2 Antibody - BSA Free

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Protocols

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