OVOL2 Antibody

Western Blot: OVOL2 Antibody [NBP2-42907] -

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (NBP2-42907) diluted at 1:500.

Western Blot: OVOL2 Antibody [NBP2-42907] -

HCT-116 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody (NBP2-42907) diluted at 1:1000.

Western Blot: OVOL2 Antibody [NBP2-42907] -

Hp. induces HNF4A silencing to activate EMT signaling in GC.A The gene expression correlation analysis showed that HNF4A-induced target genes were co-expressed with epithelial genes, but were negatively co-expressed with TGFB1 gene in TCGA_STAD. B, C The gene expression analysis showed that the epithelial genes as well as HNF4A-induced target genes were highly expressed in GC patients with Hp. positive infection. D, E Hp. infection experiments with different MOI were conducted in the AGS (highly expressed HNF4A) and MKN74 (hardly expressed HNF4A) cell lines AGS. The western blotting assays were performed to examine the effect of Hp. infection on EMT signaling in GC cell lines. F The AGS cell line with or without HNF4A overexpression was successfully constructed using lentivirus transfection. The plasmid for HNF4A overexpression was shown. In briefly, the AGS cell line without HNF4A overexpression (oe-NC) only contains endogenous HNF4A with wildtype promoter. The AGS cell line with HNF4A overexpression (oe-HNF4A) contains endogenous HNF4A with wildtype HNF4A promoter and exogenous HNF4A with Ubiquitin gene promoter. G The EMT signaling activated by Hp. infection can be restored by exogenous HNF4A overexpression. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-025-08029-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.