P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

Novus Biologicals | Catalog # NBP3-09093

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AK4

Format

Low Endotoxin
View all P-Selectin/CD62P Primary Antibodies »

Product Specifications

Immunogen

Human platelets

Specificity

The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093] - Separation of human thrombocytes (red-filled) from CD62P negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (AK4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.
Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093]

Flow Cytometry: P-Selectin/CD62P Antibody (AK4) - Low Endotoxin, Azide and BSA Free [NBP3-09093] - Surface staining pattern of human peripheral blood stained using anti-human CD62P (AK4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

Applications for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

Application
Recommended Usage

Flow Cytometry

1.5 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for P-Selectin/CD62P Antibody (AK4) - Low Endotoxin

There are currently no reviews for this product. Be the first to review P-Selectin/CD62P Antibody (AK4) - Low Endotoxin and earn rewards!

Have you used P-Selectin/CD62P Antibody (AK4) - Low Endotoxin?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies