P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free

Novus Biologicals | Catalog # NB100-65391

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Psel.KO.2.12

Format

BSA Free
View all P-Selectin/CD62P Primary Antibodies »

Product Specifications

Immunogen

CD62P transfected 300.19 cells

Reactivity Notes

Predicted cross-reactivities: Rat, Sheep, Goat

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free [NB100-65391]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free [NB100-65391]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) [NB100-65391] - Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD62P. All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum.

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human)

Gene Symbol

SELP

UniProt

P16109

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free

Certificate of Analysis

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Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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