p27/Kip1 Antibody (KIP1/9168R)

Novus Biologicals | Catalog # NBP3-23904

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KIP1/9168R
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human p27/Kip1 protein

Localization

Nucleus.

Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-i induced G1 arrest.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for p27/Kip1 Antibody (KIP1/9168R)

p27/Kip1 Antibody (KIP1/9168R) Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/9168R) [NBP3-23904] -

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/9168R) [NBP3-23904] -

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/9168R) [NBP3-23904] - IHC analysis of formalin-fixed, paraffin-embedded human prostate. Strong nuclear staining using p27/Kip1 Antibody (KIP1/9168R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for p27/Kip1 Antibody (KIP1/9168R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (KIP1/9168R)

Certificate of Analysis

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Product Specific Notices for p27/Kip1 Antibody (KIP1/9168R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail